rabbit anti nlr family pyrin domain  (Bioss)

Journal: bioRxiv

Article Title: Altered MicroRNA Signatures in Circulating Extracellular Vesicles Reflect Aortic Dilation in Takayasu Arteritis

doi: 10.1101/2025.09.21.677656

Figure Lengend Snippet: Suppressive role of miR-223-3p in NLRP3 activation (A) Predicted binding site of miR-223-3p in the 3′ UTR of NLRP3 mRNA, identified using TargetScan v8.0 and conserved across multiple mammalian species. (B) Western blot analysis of NLRP3 protein expression in THP-1-derived macrophages transfected with synthetic miR-223-3p mimics. Cells were differentiated with PMA and stimulated with LPS (One-way ANOVA). Data are presented as mean ± SD. Abbreviations: NLRP3, NOD-like receptor family pyrin domain containing 3; PMA, phorbol 12-myristate 13-acetate; LPS, lipopolysaccharide; UTR, untranslated region; SD, standard deviation

Article Snippet: Following blocking with 5% skim milk in tris-buffered saline, the membranes were incubated overnight at 4°C with primary antibodies: mouse anti-CD63 (Santa Cruz, sc-5275; 1:500), mouse anti-TSG101 (Santa Cruz, sc-7964; 1:500), rabbit anti-APOA1 (Abcam ab52945; 1:500), mouse anti-ATP5A (Abcam ab14748; 1:500), rabbit anti-NLR family pyrin domain containing 3 (NLRP3) (Cell signaling, 15101S; 1:1000), and mouse anti-GAPDH (Santa Cruz, sc-32233; 1:10000).

Techniques: Activation Assay, Binding Assay, Western Blot, Expressing, Derivative Assay, Transfection, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Celastrol attenuates human parvovirus B19 NS1‑induced NLRP3 inflammasome activation in macrophages

doi: 10.3892/mmr.2023.13080

Figure Lengend Snippet: Effects of celastrol on inflammasome signaling in macrophages. (A) Expression levels of NLRP3, ASC, caspase-1 and IL-18 proteins in U937 and THP-1 macrophages. Bar charts represent the ratios relative to the amount of GAPDH in (B) U937 and (C) THP-1 macrophages. Similar results were obtained in three repeated experiments. *P<0.05 vs. controls (0 µM); & P<0.05 vs. 1 µg/ml B19V NS1. B19V NS1. B19V, human parvovirus B19; NS1, non-structural protein 1; NLRP3, NLR family pyrin domain-containing 3; ASC, apoptosis-associated speck-like protein.

Article Snippet: The membrane was blocked with 5% non-fat dry milk in PBS for 2 h at 25°C with gentle agitation, and then incubated with antibodies against NLR family pyrin domain-containing 3 (NLRP3; 1:2,000; cat. no. A12694; ABclonal), apoptosis-associated speck-like protein (ASC; 1:2,000; cat. no. A1170; ABclonal), caspase-1 (1:2,000; cat. no. A0964; ABclonal Biotech Co., Ltd.), IL-18 (1:1,000; cat. no. 061115; MilliporeSigma) and GAPDH (1:1,000; cat. no. NB300221; Novus Biologicals, LLC) for 3 h at 25°C.

Techniques: Expressing